Therapeutic effect of organic gallium on ovariectomized osteopenic rats by decreased serum minerals and increased bone mineral content (2024)

Gallium modulates osteoclastic bone resorption in vitro without affecting osteoblasts

British Journal of Pharmacology, 2010

Distribution of trace levels of therapeutic gallium in bone as mapped by synchrotron x-ray microscopy

Proceedings of the National Academy of Sciences, 1990

Molecular effects of gallium on osteoclastic differentiation of mouse and human monocytes

Biochemical Pharmacology, 2012

A generalized increase in uptake of gallium-67 in bone

Seminars in Nuclear Medicine, 1984

A new synthetic diet increased bone mineral density and strength of ovariectomized rat

Gallic Acid Ameliorates Cadmium Effect on Osteogenesis by Activation of Alkaline Phosphatase and Collagen Synthesis

Cell J. 25(9): 603-612, 2023

Objective: We previously reported that cadmium (Cd) inhibits osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In addition, gallic acid (GA) improves BMSC differentiation. Here, we aim to study the ability of GA to prevent osteogenic inhibition induced by Cd. Materials and Methods: In this experimental study, BMSCs were extracted and purified from Wistar rats and their viability was determined in the presence of Cd and GA. The results indicated that 1.5 µM Cd and 0.25 µM of GA were appropriate for further investigation. After 20 days in osteogenic medium, matrix production was analysed by alizarin red, calcium content, and alkaline phosphatase (ALP) activity. Osteogenic-related genes and collagen 1A1 (COL1A1) protein expressions were investigated. The preventive effect of GA on oxidative stress and metabolic change induced by Cd was estimated. Results: GA counteracted the inhibitory effect of Cd on matrix production and significantly (P=0.0001) improved the osteogenic differentiation ability of BMSCs. Also, GA prevented the toxic effect of Cd on osteogenic-related gene expressions and nullified the reducing effect of Cd on COL1Al and ALP activity. A significant reduction (P=0.0001) in malondialdehyde and lactic acid concentration showed that GA counteracted both oxidative stress and metabolic changes caused by Cd. Conclusion: GA prevented the toxic effect of Cd, an environmental pollutant and a factor in osteoporosis.

Elemental distributions in femoral bone of rat under osteoporosis preventive treatments

Journal of Microscopy, 2006

The Effect of Grouper Bone Nano-Calcium (GBN) and Medium-Chain Triglyceride (MCT) Supplementation on the Ovariectomized Rats

Journal of Nutrition and Metabolism, 2024

Te objective of this research was to investigate the calcium bioavailability and the infuence of substituting synthetic calcium carbonate (CaCO3) with grouper bone nano-calcium (GBN), and medium-chain triglyceride (MCT) with long-chain triglyceride (LCT) in the diet of ovariectomized rats maintained for 8 weeks. Twenty rats were randomly divided into four distinct groups: (1) Te OX-C group: AIN-93M standard + synthetic CaCO3; (2) the OX-D group: AIN-93M standard + no calcium; (3) the OX-1 group: AIN-93M standard + GBN; and (4) the OX-2 group: AIN-93M with MCT as lipid source + GBN. Te test parameters conducted encompassed the evaluation of the rat’s body weight, levels of calcium, phosphorus, and alkaline phosphatase in rat blood serum, examination of the microstructure of rat tibiae by histomorphometry and femora bones by means of 3D micro-CT image analysis, and assessment of the strength of rat femora bones by the three-point bending. Te results indicated that the GBN calcium diet groups (OX-1 and OX-2) were successful substitutes for synthetic CaCO3 of the OX-C group. GBN calcium diet groups have shown superiority in terms of trabeculae thickness (Tb.T), bone volume (BV/TV), bone mineral density (BMD), and particularly in bone strength evaluations. Te GBN calcium diet groups exhibited serum calcium, serum phosphorus, and alkaline phosphatase levels that were comparable to those of synthetic CaCO3 calcium. As the calcium-defcient group, OX-D revealed a much lower and distinct performance than other groups. Tis research demonstrated that MCT exhibit comparable performance to LCT; however, it did not establish that substituting LCT for MCT was superior.

Evaluation of anti-osteoporosis in ovariectomized Wistar rats treated with antler blood by synchrotron radiation X-ray fluorescence microprobe

Spectroscopy, 2009

To evaluate the anti-osteoporotic effect of antler blood, the relationship between the change of element content and the bone mineral density (BMD) increase in femur was investigated. Female Wistar rats were randomly divided into three groups: sham-operated group (SHAM,n=5), ovariectomized group (OVX,n=5) and ovariectomized group with antler blood treatment (n=5). The femoral BMD was analyzed by dual-energy X-ray absorptiometry (DXA) and the element relative content was determined by synchrotron radiation X-ray fluorescence (SRXRF) microprobe. The results showed that the femoral BMD in ovariectomized rats was significantly lower than that of sham-operated rats (p<0.05) but reversed by antler blood treatment (p<0.05). A further study demonstrated that the relative contents of phosphorus (P), calcium (Ca), zinc (Zn) and strontium (Sr) were obviously lower in ovariectomized rats compared to sham-operated rats but only the relative contents of P, Ca and Zn were normalized by antle...

Gallium nitrate inhibits calcium resorption from bone and is effective treatment for cancer-related hypercalcemia

Journal of Clinical Investigation, 1984